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+.\" Title: PRIMER3_CORE
+.\" Author:
+.\" Generator: DocBook XSL Stylesheets v1.73.2 <http://docbook.sf.net/>
+.\" Date: 05/09/2008
+.\" Manual: Primer3 User Manuals
+.\" Source: primer3_core 1.1.4
+.\"
+.TH "PRIMER3_CORE" "1" "05/09/2008" "primer3_core 1.1.4" "Primer3 User Manuals"
+.\" disable hyphenation
+.nh
+.\" disable justification (adjust text to left margin only)
+.ad l
+.SH "NAME"
+primer3_core \- Designs primers for PCR
+.SH "SYNOPSIS"
+.HP 13
+\fBprimer3_core\fR [\-format_output] [\-strict_tags] [<\ \fIinput_file\fR]
+.SH "DESCRIPTION"
+.PP
+primer3_core picks primers for PCR reactions, considering as criteria oligonucleotide melting temperature, size, GC content and primer\-dimer possibilities, PCR product size, positional constraints within the source sequence, and miscellaneous other constraints\&.
+.PP
+By default, primer3_core accepts input and produces output in Boulder\-io format, a pre\-XML text\-based input/output format for program\-to\-program data interchange format\&. The Boulder\-io format and the commands that primer3_core understands are described in the
+\fIREADME\fR
+file, which on Debian systems can be found in
+\fI/usr/share/doc/primer3/\fR\&.
+.SH "OPTIONS"
+.PP
+\fB\-format_output\fR
+.RS 4
+Prints a more user\-oriented report for each sequence\&.
+.RE
+.PP
+\fB\-strict_tags\fR
+.RS 4
+primer3_core echoes and ignores any tags it does not recognize, unless the
+\fB\-strict_tags\fR
+flag is set on the command line, in which case primer3_core prints an error in the PRIMER_ERROR output tag, and prints additional information on stdout; this option can be useful for debugging systems that incorporate primer\&.
+.RE
+.sp
+.it 1 an-trap
+.nr an-no-space-flag 1
+.nr an-break-flag 1
+.br
+Note
+.PP
+The old flag \-2x_compat is no longer supported\&.
+.SH "EXIT STATUS CODES"
+.sp
+.RS 4
+\h'-04'\(bu\h'+03'0 on normal operation\&.
+.RE
+.sp
+.RS 4
+\h'-04'\(bu\h'+03'\-1 under the following conditions: illegal command\-line arguments, unable to fflush stdout, unable to open (for writing and creating) a \&.for, \&.rev or \&.int file (probably due to a protection problem)\&.
+.RE
+.sp
+.RS 4
+\h'-04'\(bu\h'+03'\-2 on out\-of\-memory\&.
+.RE
+.sp
+.RS 4
+\h'-04'\(bu\h'+03'\-3 empty input\&.
+.RE
+.sp
+.RS 4
+\h'-04'\(bu\h'+03'\-4 error in a "Global" input tag (message in PRIMER_ERROR)\&.
+.RE
+.SH "REFERENCE"
+.PP
+Please cite Rozen, S\&., Skaletsky, H\&. "Primer3 on the WWW for general users and for biologist programmers\&." In S\&. Krawetz and S\&. Misener, eds\&. Bioinformatics Methods and Protocols in the series Methods in Molecular Biology\&. Humana Press, Totowa, NJ, 2000, pages 365\-386\&.
+.SH "SEE ALSO"
+.PP
+
+\fBntdpal\fR(1)
+\fBoligotm\fR(1)
+.SH "COPYRIGHT"
+Copyright \(co 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008 Whitehead Institute for Biomedical Research, Steve Rozen (http://jura.wi.mit.edu/rozen), Helen Skaletsky
+.br
+.PP
+All rights reserved\&. On Debian\-based systems, please consult
+\fI/usr/share/doc/primer3/copyright\fR
+to read the licence of primer3_core\&.
+.PP
+This manual page was written by Steffen Moeller
+<moeller@debian\&.org>
+for the
+Debian(TM)
+system (but may be used by others)\&. Permission is granted to copy, distribute and/or modify this document under the same terms as primer3_core itself\&.
+
+.sp